Invitrogen nupage gel manual

Invitrogen™ NuPAGE™ 4 to 12%, Bis-Tris, mm, Mini Protein Gel, well Separation Range: to kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini Brand: Invitrogen™ NPBOX. It consists of nine proteins, ranging in size from 40 to kDa in NuPAGE Novex % and 7% Tris-Acetate Gel/SDS buffer systems. Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. Several NuPAGE Large Protein Starter Kits are available to provide you with all necessary reagents and optimized.  · Technical tips. In the Invitrogen NuPAGE Bis-Tris discontinous buffer system, the electrophoretic mobility of the proteins and the subsequent separation range of the gel is dependent on two factors: i) the acrylamid concentration of the gel (with greater acrylamide concentration resulting in better resolution of lower molecular weight proteins) and ii) the trailing ion of the running buffer.

Choose the right NuPAGE™ Novex™ Bis-Tris Gel for your protein separation. Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE™ Novex™ Bis-Tris mini gels come in 3 polyacrylamide concentrations: 10%, 12%, and a 4–12% gradient. We also offer 8% gels in the midi-gel format. Simplified instructions for Invitrogen NuPAGE Gels Running NuPAGE Gels 1. Select the desired Running Buffer (MOPS works for to 14 kDa and MES for 60 to kDa) and make up ml using the 20X stocks stored at 4 degrees. 2. Remove precast gel from bag, rinse with water. Peel off tape on back of gel and remove comb. Protein separation comparing NuPAGE Bis-Tris Gel and traditional tris-glycine gel. The samples listed below were run on (A) a NuPAGE 4–12% Bis-Tris Gel in Invitrogen NuPAGE MES SDS Running Buffer or (B) another manufacturer’s 4–20% tris-glycine gel. Note the high resolution of the sample bands in the NuPAGE protein gel.

Instructions are provided below to prepare samples for non-denaturing (native) gel electrophoresis using the NuPAGE® Novex® Tris-Acetate Gels. Reagent. Volume. GenScript Express PAGE Gels is a high performance precast polyacrylamide gel system with a variety of acrylamide GenScript Precast Gel Selection Guide. additional details on loading volumes and maximum protein load, refer to the Novex® Pre-Cast Gel. Electrophoresis Guide or the NuPAGE® Technical. Guide. Gel.